Indlela yokubona i-Ca2+:
Izixhobo zovavanyo kunye nee-reagents: ii-beakers; iflaski yekhonikhi; iFunnel; iBurette; i-Electric furnace; i-Anhydrous ethanol; i-Hydrochloric acid, isisombululo se-NH3-NH4Cl buffer, i-calcium indicator, isisombululo esiqhelekileyo se-0.02mol/LEDTA.
Amanyathelo ovavanyo:
1. Linganisa ngokuchanekileyo ubungakanani obuthile besampulu yoncedo lokucubungula i-ACR (ichaneke ukuya kwi-0.0001g) uze uyibeke kwisitya sokubhaka. Yimanzise nge-ethanol engenamanzi, uze wongeze i-hydrochloric acid engaphezulu kwe-1:1 uze uyifudumale kwisithando sombane ukuze i-calcium ions ikwazi ukusabela ngokupheleleyo kwi-hydrochloric acid;
2. Hlamba ngamanzi uze uhluze nge-funnel ukuze ufumane ulwelo olucacileyo;
3. Lungisa ixabiso le-pH libe ngaphezulu kwe-12 usebenzisa isisombululo se-NH3-NH4Cl se-buffer, yongeza isixa esifanelekileyo se-calcium indicator, kwaye udibanise isisombululo esiqhelekileyo se-0.02mol/LEDTA. Isiphelo kuxa umbala utshintsha ukusuka kubomvu obomvu ukuya kuluhlaza okwesibhakabhaka;
4. Yenza uvavanyo olungenanto ngaxeshanye;
5. Bala u-C # a2+=0.02 $(V-V0) $0.04004M $%&&
V - Umthamo wesisombululo se-EDTA esisetyenziswa xa kuvavanywa iisampulu zoncedo lokucubungula i-ACR (mL).
V # – Umthamo wesisombululo esisetyenzisiweyo ngexesha lovavanyo olungenanto
M — Linganisa ubunzima besampulu yoncedo lokucubungula i-ACR (g).
Indlela yokutshisa yokulinganisa izinto ezingaphiliyo:
Izixhobo zovavanyo: ibhalansi yohlalutyo, isithando somlilo esithambileyo.
Amanyathelo ovavanyo: Thabatha iisampulu zoncedo lokucubungula i-ACR eziyi-0.5,1.0g (ezichanekileyo ukuya kwi-0.001g), uzibeke kwisithando somlilo esingenamaqondo obushushu angama-950 uze uzitshise iyure e-1, uze uzipholise uze uzilinganisele ukuze ubale izinto ezitshileyo eziseleyo. Ukuba izinto ezingaphiliyo zongezwa kwisampulu yoncedo lokucubungula i-ACR, kuya kubakho intsalela engaphezulu.
Indlela yesinyibilikisi:
1. Izixhobo zovavanyo kunye nebhalansi yohlalutyo lwe-reagent; iflaski yevolumu engama-25mL; iTrichloromethane.
Inyathelo lesi-2: Ngokwendlela yokuvavanya i-ACR intrinsic viscosity, linganisa i-75mg yesampulu uze uyibeke kwiflaski ye-volumetric ye-25mL. Yongeza i-trichloromethane solvent. Ukuba kukho i-turbidity okanye ukukhanya okubonakalayo
Ixesha leposi: Meyi-27-2024
